Stat1 chip
Web10144-2-AP targets STAT1 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. WebSTAT3 belongs to the STAT family of transcription factors consisting of seven proteins, namely, STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b and STAT6. Activated STAT3 translocates into the cell nucleus and binds to the interferon-gamma activated sequence ... STAT1: ChIP: −604 to −596 bp −444 to −435 bp −363 to −356 bp −246 to − ...
Stat1 chip
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WebStat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate ... Supershift and ChIP applications, sc-8394 X, 200 µg/0.1 ml. p-Stat1 (A-2) is available conjugated to agarose (sc-8394 AC), 500 µg/0.25 ml WebMar 4, 2016 · Columns depict STAT1 ChIP-seq data from mouse bone marrow–derived macrophages (BMM) that were either untreated or treated with IFNB or IFNG. Only RLTR30B elements that are bound by STAT1 upon IFNG treatment are shown. Bottom metaprofiles represent average normalized ChIP signal across bound elements.
WebMar 26, 2013 · A Comprehensive Profile of ChIP-Seq-Based STAT1 Target Genes Suggests the Complexity of STAT1-Mediated Gene Regulatory Mechanisms A Comprehensive … WebJun 1, 2024 · (A) Phosphorylated STAT1 (p-STAT1), STAT1, and RBM4 protein expression in si-RBM4 transfected and IFN-γ-treated RAW264.7 macrophages tested by western blot …
WebChIP1: HeLa cells. General notes This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility …
WebSTAT3 belongs to the STAT family of transcription factors consisting of seven proteins, namely, STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b and STAT6. Activated STAT3 …
WebNov 26, 2024 · The C-terminal transactivation domain of STAT1 is required for an efficient recruitment of the components of the core Mediator complex to the IRF1 promoter, which harbors an open chromatin state under basal conditions, inducing transcriptional activation . Using ChIP assays, we found that IRF1 binds to the Mda5 promoter. the silver trade routeWebMay 15, 2024 · Of the 34 loci known to contain STAT1 interferon-responsive binding sites, ChIP-seq found 24 (71%). ChIP-seq targets were enriched in sequences similar to known STAT1 binding motifs. Comparisons with two ChIP-PCR data sets suggested that ChIP-seq sensitivity was between 70% and 92% and specificity was at least 95%. Use ChIP-seq to … my universe: pet clinic nintendo switchWebJan 5, 2011 · Basal levels of ∼25% (119/461) of these genes were found to be reduced by ≥3-fold in stat1 −/− MEFs compared to the levels for controls. Of these stringently selected genes, the expression patterns of 50 genes whose basal levels were most disrupted in the absence of STAT1 are shown in Fig. Fig.1B 1B (right panel). We defined these genes ... my university bookWebThe Stat1 Antibody confirms silencing of Stat1 expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Show More. Western blot … the silver tree bridgwaterWebOct 4, 2024 · Chromatin immunoprecipitation (ChIP)-PCR and luciferase assays were used to detect the transcriptional activity of target genes of E2F2. Nuclear translocation of STAT1 and p65 were assayed by Western blot, co-immunoprecipitation (co-IP), and immunofluorescence experiments. my universe will never be the same songWebMay 11, 2024 · Chromatin immunoprecipitation (ChIP) of STAT1-associated DNA in RAW 264.7 macrophages revealed this region, located ~800 base pairs downstream of exon 1 … my university canada west portalWebChIP1: HeLa cells. General notes This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free production For more information see here. the silver tree inn