In frame with enterokinase site
Webb• Enterokinase cleavage site for removal of fusion tag A set of three vectors is provided (A, B, and C). Each has the N-terminal tag coding sequence in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene. 效果验证 客户文章 相关产品 WebbEnterokinase, a two-chain duodenal serine protease, activates trypsinogen by removing its N-terminal propeptide. Due to a clean cut after the non-primed site recognition …
In frame with enterokinase site
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WebbpET-30a(+) Search Bacterial vector for expression of N-terminally S-tagged proteins with an enterokinase site. For other reading frames, use pET‑30b(+) or pET‑30c(+). WebbEnterokinase from bovine intestine Catalog Number E5144 Storage Temperature –20 °C CAS RN 9014-74-8 EC 3.4.21.9 Synonym: Enteropeptidase Product Description …
WebbCatalog number: E18001. EKMax™ is a recombinant preparation of the catalytic subunit of bovine enterokinase (1). EKMax™ recognizes the sequence Asp-Asp-Asp-Asp-Lys … Webbenterokinase本身的效率还是比较高的，对反应环境的要求也比较温和。. 根据NEB上的说明。. It will sometimes cleave at other basic residues， depending on the …
Webb1 sep. 1995 · The EKL cDNA was fused in-frame to the 3′-end of the coding sequence for DsbA, with the two domains of the fusion protein separated by a linker sequence … WebbDeficiency of enterokinase, a sequence-specific protease that activates trypsinogen (see 276000) and has a major role in protein digestion, is an autosomal recessive disorder …
WebbEnterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni 2+ affinity chromatography and was designed for removing from …
Webb14 dec. 2024 · Protein samples (125 μg) were mixed with 5 µL of enterokinase (New England Biolabs, Ipswich, MA, USA) in 100 µL of reaction buffer (20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl 2, pH 8.0) and left at room temperature for 16 h, … limited hydro flaskWebbThe present invention relates to fusion proteins comprising a non-cytotoxic protease and a EGF mutein ligand. The EGF mutein provides improved EGF receptor activation for the clai hotels near royals stadium kansas cityWebbEnterokinase bovine, recombinant expressed in E. coli Catalog Number E4906 Storage Temperature –20 °C CAS RN 9014-74-8 EC 3.4.21.9 Synonym: Enteropeptidase … limited hypercoagulable testingWebbthrombin site：Thrombin蛋白酶切位点，蛋白序列为LVPRGS，用凝血酶处理纯化的蛋白可以将融合蛋白的目的蛋白和分目的蛋白分开。 T7 tag：T7噬菌体编码的蛋白p10前导序 … hotels near rt 81 wvWebb1. A composition comprising: (i) an isolated Ct-858 Chlamydia protein consisting of SEQ ID NO: 6; (ii) an isolated Ct-089 Chlamydia protein consisting of SEQ ID NO:16; and (iii) a pharmaceutically acceptable carrier. hotels near rsw airport with shuttleWebb2 mars 2011 · As depicted in Figure 1a, the FP was designed as a molecular bridge to link biotinylated molecules, such as antigens and antibodies, to the RBC membrane. We analyzed binding of the FP to the surface membrane of murine RBCs in vitro using flow cytometry, labeling the FP with biotinylated fluorescein. hotels near ruffled feathers golf clubWebb名称： BPSV40-NLS. 描述：A classical nuclear localization signal (NLS) consists of two clusters of basic residues separated by 10–12 residues (bipartite), NLS-containing … limited hyundai